Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18242-1-AP targets CHST3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13008 Product name: Recombinant human CHST3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 46-479 aa of BC093690 Sequence: SRVSDKLKQIPQALADANSTDPALILAENASLLSLSELDSAFSQLQSRLRNLSLQLGVEPAMEAAGEEEEEQRKEEEPPRPAVAGPRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLWHIERTVSFEPGGANAAGSALVYRDVLKQLFLCDLYVLEHFITPLPEDHLTQFMFRRGSSRSLCEDPVCTPFVKKVFEKYHCKNRRCGPLNVTLAAEACRRKEHMALKAVRIRQLEFLQPLAEDPRLDLRVIQLVRDPRAVLASRMVAFAGKYKTWKKWLDDEGQDGLREEEVQRLRGNCESIRLSAELGLQQPAWLRGRYMLVRYEDVARGPLQKAREMYRFAGIPLTPQVEDWIQKNTQAAHDGSGIYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPAMRLFGYKLARDAAALTNRSVSLLEERGTFWVT Predict reactive species |
| Full Name | carbohydrate (chondroitin 6) sulfotransferase 3 |
| Calculated Molecular Weight | 479 aa, 55 kDa |
| Observed Molecular Weight | 55-67 kDa |
| GenBank Accession Number | BC093690 |
| Gene Symbol | CHST3 |
| Gene ID (NCBI) | 9469 |
| RRID | AB_10694672 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7LGC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHST3 antibody 18242-1-AP | Download protocol |
| WB protocol for CHST3 antibody 18242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-CHST3 antibody (Cat# 18242-1-AP) has 7 total citations published in journals including Osteoarthritis Cartilage, Cell Mol Life Sci, Cells, J Neurochem, Biol Trace Elem Res, J Trace Elem Med Biol, and Histochem Cell Biol. Research fields span osteoarthritis and Kashin-Beck disease, Golgi enzyme secretion, aging-associated cognitive changes, cerebral cortex injury, selenium deficiency effects on chondrocytes, and cartilage sulfotransferase expression in growing rats. Applications include IHC, WB, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Osteoarthritis Cartilage Altered expression of chondroitin sulfate structure modifying sulfotransferases in the articular cartilage from adult osteoarthritis and Kashin-Beck disease. | ||
Cell Mol Life Sci N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion. | ||
Cells Aging-Associated Changes in Cognition, Expression and Epigenetic Regulation of Chondroitin 6-Sulfotransferase Chst3. | ||
J Neurochem OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex. | ||
Biol Trace Elem Res Serious Selenium Deficiency in the Serum of Patients with Kashin-Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes. | ||
J Trace Elem Med Biol The effects of long-term low selenium diet on the expression of CHST-3, CHST-12 and UST in knee cartilage of growing rats |
