|Positive WB detected in||MCF-7 cells, L02 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
12287-1-AP targets CIDEC in WB, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CIDEC fusion protein Ag2913|
|Full Name||cell death-inducing DFFA-like effector c|
|Calculated molecular weight||238 aa, 27 kDa|
|Observed molecular weight||27-35 kDa|
|GenBank accession number||BC016851|
|Gene ID (NCBI)||63924|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cell death-inducing DFF45-like effector (CIDE) proteins, including CIDEA, CIDEB and CIDEC (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. CIDEC was found to be highly expressed in adipose tissues and to function as a lipid droplet-binding protein that promotes lipid accumulation in adipocytes. While normal mouse livers express low levels of CIDEC protein, its expression is highly upregulated in NALFD, but not in chronic ethanol feeding-induced fatty liver. (PMID: 26099526) Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).
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