CIDEC Polyclonal antibody

CIDEC Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12287-1-AP

Synonyms

Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 12287-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12287-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12287-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

12287-1-AP targets CIDEC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIDEC fusion protein Ag2913
• Full Name: cell death-inducing DFFA-like effector c
• Calculated molecular weight: 238 aa, 27 kDa
• Observed molecular weight: 29 kDa, 48 kDa
• GenBank accession number: BC016851
• Gene symbol: CIDEC
• Gene ID (NCBI): 63924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death-inducing DFF45-like effector (CIDE) proteins, including Cidea, Cideb and Cidec (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. Recent studies found that overexpression of Fsp27 induces the accumulation of lipid droplets (LDs) and enhances LD size; knockdown of Fsp27 decreases LD size and increases LD number in 3T3-L1 adipocytes. Cidec is localized on the surface of lipid droplets and could promote the differentiation of human adipocytes. Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).

Publications

Species	Application	Title
human	WB	Commun Biol; Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression.; Authors - Yi Jin
human	WB	Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen