CIDEC Polyclonal antibody

CIDEC Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12287-1-AP

Synonyms

Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12287-1-AP targets CIDEC in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIDEC fusion protein Ag2913
Full Name cell death-inducing DFFA-like effector c
Calculated molecular weight 238 aa, 27 kDa
Observed molecular weight 29 kDa, 48 kDa
GenBank accession numberBC016851
Gene symbol CIDEC
Gene ID (NCBI) 63924
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death-inducing DFF45-like effector (CIDE) proteins, including Cidea, Cideb and Cidec (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. Recent studies found that overexpression of Fsp27 induces the accumulation of lipid droplets (LDs) and enhances LD size; knockdown of Fsp27 decreases LD size and increases LD number in 3T3-L1 adipocytes. Cidec is localized on the surface of lipid droplets and could promote the differentiation of human adipocytes. Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).

Protocols

Product Specific Protocols
WB protocol for CIDEC antibody 12287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression.

Authors - Yi Jin
humanWB

Biomed Pharmacother

Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.

Authors - Xingran Chen