|Positive WB detected in||MCF-7 cells, HeLa cells, L02 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
12287-1-AP targets CIDEC in WB,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CIDEC fusion protein Ag2913|
|Full Name||cell death-inducing DFFA-like effector c|
|Calculated molecular weight||238 aa, 27 kDa|
|Observed molecular weight||29 kDa, 48 kDa|
|GenBank accession number||BC016851|
|Gene ID (NCBI)||63924|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cell death-inducing DFF45-like effector (CIDE) proteins, including Cidea, Cideb and Cidec (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. Recent studies found that overexpression of Fsp27 induces the accumulation of lipid droplets (LDs) and enhances LD size; knockdown of Fsp27 decreases LD size and increases LD number in 3T3-L1 adipocytes. Cidec is localized on the surface of lipid droplets and could promote the differentiation of human adipocytes. Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).