CIDEC Polyclonal antibody
CIDEC Polyclonal Antibody for WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 12287-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HeLa cells, L02 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
12287-1-AP targets CIDEC in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CIDEC fusion protein Ag2913 |
Full Name | cell death-inducing DFFA-like effector c |
Calculated molecular weight | 238 aa, 27 kDa |
Observed molecular weight | 29 kDa, 48 kDa |
GenBank accession number | BC016851 |
Gene symbol | CIDEC |
Gene ID (NCBI) | 63924 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cell death-inducing DFF45-like effector (CIDE) proteins, including Cidea, Cideb and Cidec (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. Recent studies found that overexpression of Fsp27 induces the accumulation of lipid droplets (LDs) and enhances LD size; knockdown of Fsp27 decreases LD size and increases LD number in 3T3-L1 adipocytes. Cidec is localized on the surface of lipid droplets and could promote the differentiation of human adipocytes. Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).
Protocols
Product Specific Protocols | |
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WB protocol for CIDEC antibody 12287-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Commun Biol Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression. | ||
Biomed Pharmacother Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway. |