CIDEC Polyclonal antibody

CIDEC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12287-1-AP

Synonyms

Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27

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Validation Data Gallery

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  WB analysis of MCF-7 using 12287-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12287-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

12287-1-AP targets CIDEC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIDEC fusion protein Ag2913
• Full Name: cell death-inducing DFFA-like effector c
• Calculated Molecular Weight: 238 aa, 27 kDa
• Observed Molecular Weight: 27-35 kDa
• GenBank Accession Number: BC016851
• Gene Symbol: CIDEC
• Gene ID (NCBI): 63924
• RRID: AB_2877842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell death-inducing DFF45-like effector (CIDE) proteins, including CIDEA, CIDEB and CIDEC (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. CIDEC was found to be highly expressed in adipose tissues and to function as a lipid droplet-binding protein that promotes lipid accumulation in adipocytes. While normal mouse livers express low levels of CIDEC protein, its expression is highly upregulated in NALFD, but not in chronic ethanol feeding-induced fatty liver. (PMID: 26099526) Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).

Publications

Species	Application	Title
mouse	WB	J Nutr Biochem; Identification of potential pathogenic hepatic super-enhancers regulatory network in high-fat diet induced hyperlipidemia; Authors - Yingying Hu
human	WB	Commun Biol; Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression.; Authors - Yi Jin
human	WB	Cancer Biol Ther; The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer; Authors - Wen-Jing Yun
human	WB	Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen
mouse	WB	Aging Cell; Long-term NAD+ supplementation prevents the progression of age-related hearing loss in mice; Authors - Mustafa N Okur
mouse	WB	Cell Death Discov; Shift work promotes adipogenesis via cortisol-dependent downregulation of EGR3-HDAC6 pathway; Authors - Xinxing Wan