CIN85 Monoclonal antibody, PBS Only

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  WB analysis using 66571-1-Ig (same clone as 66571-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66571-1-Ig (same clone as 66571-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 66571-1-Ig (same clone as 66571-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66571-1-Ig (same clone as 66571-1-PBS)

  rat brain and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.

CIN85 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66571-1-PBS

Clone No.3C12D2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1

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Product Information

66571-1-PBS targets CIN85 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2777

  Product name: Recombinant human CIN85 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-328 aa of BC015806

  Sequence: MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPP

• Full Name: SH3-domain kinase binding protein 1
• Calculated Molecular Weight: 665 aa, 73 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC015806
• Gene Symbol: SH3KBP1/CIN85
• Gene ID (NCBI): 30011
• RRID: AB_2881932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96B97
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.