CIP2A Polyclonal antibody

• Featured Product
• KD/KO Validated

CIP2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23199-1-AP

Synonyms

Cancerous inhibitor of PP2A, CIP2A, KIAA1524, p90, p90 autoantigen, Protein CIP2A

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Validation Data Gallery

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  WB analysis using 23199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23199-1-AP (KIAA1524 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23199-1-AP

  WB result of CIP2A antibody (23199-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CIP2A transfected A549 cells.

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  IHC staining of human lung cancer using 23199-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 23199-1-AP (CIP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 23199-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23199-1-AP (CIP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, U2OS cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23199-1-AP is CIP2A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CIP2A fusion protein Ag19598
• Full Name: KIAA1524
• Calculated Molecular Weight: 905 aa, 102 kDa
• Observed Molecular Weight: 80-102 kDa
• GenBank Accession Number: BC130564
• Gene Symbol: KIAA1524
• Gene ID (NCBI): 57650
• RRID: AB_2918079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)