Featured Product
KD/KO Validated

CKAP2L Polyclonal antibody

CKAP2L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17143-1-AP

Publications (4)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis using 17143-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17143-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17143-1-AP (CKAP2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of Jurkat using 17143-1-AP
IP result of anti-CKAP2L (IP:17143-1-AP, 4ug; Detection:17143-1-AP 1:2000) with Jurkat cells lysate 1560 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse lung using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat colon using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat lung using 17143-1-AP
Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 17143-1-AP (CKAP2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 17143-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP2L antibody (17143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CKAP2L in ?

Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human colon cancer, rat lung tissue, rat colon tissue, mouse lung tissue, human placenta tissue, human ovary cancer tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17143-1-AP targets CKAP2L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10909 Product name: Recombinant human CKAP2L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 396-745 aa of BC036217 Sequence: IRPNGTSGNKHNNNGFQQKAQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQTNPNIKKKATAEDRRKQLEEWQKSKGKTYKRPPMELKTKRKVIKEMNISFWKSIEKEEEEKKAQLELSSKINNTLTECLNLIEGGVPSNEILNILSSIPEAEKFAKFWICKAKLLASKGTFDVIGLYEEAIKNGATPIQELRKVVLNILQDSNRTTEGITSDSLVAETSITSVEELAKKMESVKSCLSPKEREQVTATPRIAKAEQHNYPGIKLQIGPIPRINGMPEVQDMKFITPVRRSSRIERAVSRYPEMLQEHDLVVASLDELLEVEETKCFIFRRNEALPVTLGFQTPES Predict reactive species
Full Name	cytoskeleton associated protein 2-like
Calculated Molecular Weight	745 aa, 84 kDa
Observed Molecular Weight	84 kDa
GenBank Accession Number	BC036217
Gene Symbol	CKAP2L
Gene ID (NCBI)	150468
RRID	AB_2080720
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IYA6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).

Protocols

Product Specific Protocols
IF protocol for CKAP2L antibody 17143-1-AP	Download protocol
IHC protocol for CKAP2L antibody 17143-1-AP	Download protocol
IP protocol for CKAP2L antibody 17143-1-AP	Download protocol
WB protocol for CKAP2L antibody 17143-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The anti-CKAP2L antibody (Cat# 17143-1-AP) has 4 total citations published in J Transl Med, Cell Reports, FASEB J, and Int J Clin Exp Pathol. Research fields span bladder cancer progression, radial glial ciliary protein mapping in telencephalon development, ovarian cancer metastasis via m6A-dependent regulation, and hepatocellular carcinoma prognosis. Applications cited include WB, IF, and IHC in human and mouse samples.

Species	Application	Title
human	WB	J Transl Med Smoking promotes the progression of bladder cancer through FOXM1/CKAP2L axis Authors - Feixiang Wu View Article
mouse	IF	Cell Rep Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalon. Authors - Xiaoliang Liu View Article
human	WB,IHC	FASEB J IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6 A-dependent manner Authors - Yaqian Shi View Article
mouse	WB	Int J Clin Exp Pathol Oncogenic and prognostic role of CKAP2L in hepatocellular carcinoma. Authors - Penghui Wang KD Validated View Article