CKAP2L Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86247-1-RR (same clone as 86247-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86247-1-RR (CKAP2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 86247-1-RR (same clone as 86247-1-PBS)

  IP result of anti-CKAP2L (IP:86247-1-RR, 4ug; Detection:86247-1-RR 1:1000) with Jurkat cells lysate 1360 ug. This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86247-1-RR (same clone as 86247-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP2L antibody (86247-1-RR, Clone: 250860G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86247-1-PBS in a different storage buffer formulation.

CKAP2L Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 86247-1-PBS

Clone No.250860G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Cytoskeleton-associated protein 2-like, Radial fiber and mitotic spindle protein, Radmis

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Product Information

86247-1-PBS targets CKAP2L in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10909

  Product name: Recombinant human CKAP2L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 396-745 aa of BC036217

  Sequence: IRPNGTSGNKHNNNGFQQKAQTLDSKLKKAVPQNHFLNKTAPKTQADVTTVNGTQTNPNIKKKATAEDRRKQLEEWQKSKGKTYKRPPMELKTKRKVIKEMNISFWKSIEKEEEEKKAQLELSSKINNTLTECLNLIEGGVPSNEILNILSSIPEAEKFAKFWICKAKLLASKGTFDVIGLYEEAIKNGATPIQELRKVVLNILQDSNRTTEGITSDSLVAETSITSVEELAKKMESVKSCLSPKEREQVTATPRIAKAEQHNYPGIKLQIGPIPRINGMPEVQDMKFITPVRRSSRIERAVSRYPEMLQEHDLVVASLDELLEVEETKCFIFRRNEALPVTLGFQTPES

• Full Name: cytoskeleton associated protein 2-like
• Calculated Molecular Weight: 745 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC036217
• Gene Symbol: CKAP2L
• Gene ID (NCBI): 150468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IYA6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CKAP2L is a microtubule-binding protein that is crucial for the formation of the mitotic spindle and the cell cycle progression of neural progenitor cells. Mutations in CKAP2L have been associated with spindle organization defects, including mitotic spindle defects, lagging chromosomes, and chromatin bridges. There is evidence that mutations in this gene are associated with Filippi syndrome, characterized by growth defects, microcephaly, intellectual disability, facial feature defects, and syndactyly(PMID: 25439729).