Product Information
13713-1-AP targets CLASP1 in ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4651 Product name: Recombinant human CLASP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1390-1538 aa of BC032563 Sequence: MKTLEAHKDSHKEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERIAKESLLQLLVDIIPGLLQGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS Predict reactive species |
| Full Name | cytoplasmic linker associated protein 1 |
| Calculated Molecular Weight | 1538 aa, 169 kDa |
| GenBank Accession Number | BC032563 |
| Gene Symbol | CLASP1 |
| Gene ID (NCBI) | 23332 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z460 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLASP1 and CLASP2 are homologous mammalian proteins, which associate with the ends of growing microtubules. In interphase cells CLASPs can attach microtubule plus ends to the cortex and stabilize them by repeatedly rescuing them from depolymerization. A truncated form of CLASP1 lacking the kinetochore binding domain behaves as a dominant negative, leading to the formation of radial arrays of microtubule bundles that are highly resistant to depolymerization. Microinjection of CLASP1-specific antibodies suppresses microtubule dynamics at kinetochores and throughout the spindle, resulting in the formation of monopolar asters with chromosomes buried in the interior. Therefore, CLASP1 is required at kinetochores for attached microtubules to exhibit normal dynamic behavior.
