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CLCC1 Polyclonal antibody

CLCC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26680-1-AP

Synonyms

chloride channel CLIC like 1, CLCC1, KIAA0761, MCLC

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using CLCC1 Polyclonal antibody (26680-1-AP)

    WB analysis of COLO 320 using 26680-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26680-1-AP (CLCC1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26680-1-AP targets CLCC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCC1 fusion protein Ag24682
Full Name chloride channel CLIC-like 1
Calculated Molecular Weight 551 aa, 62 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC002939
Gene Symbol CLCC1
Gene ID (NCBI) 23155
RRIDAB_2880600
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLCC1, also known as MCLC, is a transmembrane protein that localizes to intracellular compartments, including endoplasmic reticulum (ER), and it functions as a chloride channel (PMID: 11279057). It has been shown that loss of CLCC1 results in ER Stress, misfolded protein accumulation, and neurodegeneration (PMID: 25698737). CLCC1 forms homomultimer in the ER membrane and is a pore-forming component of an anion channel. Studies found that disruption of ER ion homeostasis maintained by CLCC1 is one of the causes of neurodegenerative diseases, such as ALS (amyotrophic lateral sclerosis) (PMID: 37142673).

Protocols

Product Specific Protocols
WB protocol for CLCC1 antibody 26680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Yu-Ping (Verified Customer) (05-04-2020)

This product performed well compared with the product from other company

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cell line