CLCNKA Polyclonal antibody

CLCNKA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14402-1-AP

Synonyms

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka



Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, mouse pancreas tissue, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14402-1-AP targets CLCNKA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCNKA fusion protein Ag5647
Full Name chloride channel Ka
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC048282
Gene Symbol CLCNKA
Gene ID (NCBI) 1187
RRIDAB_2079857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLCNKA antibody 14402-1-APDownload protocol
IHC protocol for CLCNKA antibody 14402-1-APDownload protocol
IP protocol for CLCNKA antibody 14402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols