CLCNKA Polyclonal antibody

CLCNKA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14402-1-AP

Synonyms

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka

---

Validation Data Gallery

• 

  WB analysis using 14402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14402-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14402-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14402-1-AP

  IP result of anti-CLCNKA (IP:14402-1-AP, 5ug; Detection:14402-1-AP 1:500) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human kidney using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, human kidney tissue, mouse pancreas tissue, mouse kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14402-1-AP targets CLCNKA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLCNKA fusion protein Ag5647
• Full Name: chloride channel Ka
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC048282
• Gene Symbol: CLCNKA
• Gene ID (NCBI): 1187
• RRID: AB_2079857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

NEDD4L Polyclonal antibody

13690-1-AP

CLCA2 Polyclonal antibody

19273-1-AP

CLIC1 Polyclonal antibody

14545-1-AP