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Claudin 22 Polyclonal antibody

Claudin 22 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20724-1-AP

Synonyms

claudin 22, CLDN22

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Claudin 22 Polyclonal antibody (20724-1-AP)

    WB analysis of HeLa using 20724-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20724-1-AP (Claudin 22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Claudin 22 Polyclonal antibody (20724-1-AP)

    WB analysis of A431 using 20724-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20724-1-AP (Claudin 22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20724-1-AP targets Claudin 22 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name claudin 22
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberNM_001111319
Gene Symbol CLDN22
Gene ID (NCBI) 53842
RRIDAB_2878727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLDN22 belongs to the claudin family. It plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. The antibody is specific to CLD22.

Protocols

Product Specific Protocols
WB protocol for Claudin 22 antibody 20724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols