SCGF Monoclonal antibody

SCGF Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

4H11E4

Cat no : 60295-1-Ig

Synonyms

CLEC11A, CLECSF3, LSLCL, P47, SCGF, Stem cell growth factor



Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60295-1-Ig targets SCGF in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SCGF fusion protein Ag21365
Full Name C-type lectin domain family 11, member A
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC005810
Gene symbol CLEC11A
Gene ID (NCBI) 6320
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.

Protocols

Product Specific Protocols
WB protocol for SCGF antibody 60295-1-IgDownload protocol
IHC protocol for SCGF antibody 60295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC, IF

FASEB J

The "Matrisome" reveals the characterization of skin keloid microenvironment.

Authors - Shikun Zhang