Filter: AllWBIHCIF-PIF/ICC

WB analysis using 60295-1-Ig (same clone as 60295-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60295-1-Ig (SCGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 60295-1-Ig (same clone as 60295-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 60295-1-Ig (CLEC11A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.
IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.
IF Staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 60295-1-Ig (same clone as 60295-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

SCGF Monoclonal antibody, PBS Only

SCGF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 60295-1-PBS

Clone No.4H11E4

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60295-1-PBS targets SCGF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag21365 Product name: Recombinant human CLEC11A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-323 aa of BC005810 Sequence: GHGARGAEREWEGGWGGAQEEEREREALMLKHLQEALGLPAGRGDENPAGTVEGKEDWEMEEDQGEEEEEEATPTPSSGPSPSPTPEDIVTYILGRLAGLDAGLHQLHVRLHALDTRVVELTQGLRQLRNAAGDTRDAVQALQEAQGRAEREHGRLEGCLKGLRLGHKCFLLSRDFEAQAAAQARCTARGGSLAQPADRQQMEALTRYLRAALAPYNWPVWLGVHDRRAEGLYLFENGQRVSFFAWHRSPRPELGAQPSASPHPLSPDQPNGGTLENCVAQASDDGSWWDHDCQRRLYYVCEFPF Predict reactive species
Full Name	C-type lectin domain family 11, member A
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36-40 kDa
GenBank Accession Number	BC005810
Gene Symbol	SCGF
Gene ID (NCBI)	6320
RRID	AB_2881410
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9Y240
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.