Anti-Human CLEC12A Rabbit Recombinant Antibody

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• Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR)

CLEC12A Uni-rAb^® Recombinant Antibody for FC

Cat No. 98083-1-RR Copied!

Clone No. 240791B7

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

240791B7, C type lectin like molecule 1, CD371, CLL 1, CLL1

• 

  FC experiment of human peripheral blood leukocytes using 98083-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human peripheral blood leukocytes using 98083-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7)(red), or 0.25 ug Isotype Control(blue) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining.Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human peripheral blood leukocytes using 98083-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or other vendor.Cells were then stained with CoraLite® Plus 647 Anti-Human CD16. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98083-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98083-1-RR against Human CLEC12A were performed. The affinity constant is 0.524 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98083-1-RR targets CLEC12A in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0024

  Product name: Recombinant Human CLEC12A protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: N-6*his

  Domain: 65-265 aa of BC126291

  Sequence: HVTLKIEMKKMNKLQNISEELQRNISLQLMSNMNISNKIRNLSTTLQTIATKLCRELYSKEQEHKCKPCPRRWIWHKDSCYFLSDDVQTWQESKMACAAQNASLLKINNKNALEFIKSQSRSYDYWLGLSPEEDSTRGMRVDNIINSSAWVIRNAPDLNNMYCGYINRLYVQYYHCTYKQRMICEKMANPVQLGSTYFREA

• Full Name: C-type lectin domain family 12, member A
• Calculated Molecular Weight: 31 kDa
• GenBank Accession Number: BC126291
• Gene Symbol: CLEC12A
• Gene ID (NCBI): 160364
• RRID: AB_3672230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5QGZ9
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).