CoraLite®594 Anti-Human CLEC12A Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using CL594-98083

  1x10^6 human peripheral blood leukocytes were surface stained with FITC Plus Anti-Human CD16, and 5 ul CoraLite®594 Anti-Human CLEC12A Rabbit RecAb (CL594-98083, Clone: 240791B7) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL594-98083

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite®594 Anti-Human CLEC12A Rabbit RecAb (CL594-98083, Clone: 240791B7) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

CLEC12A Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL594-98083

Clone No.240791B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

240791B7, C type lectin like molecule 1, CD371, CLL 1, CLL1

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98083 targets CLEC12A in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLEC12A fusion protein Eg0024
• Full Name: C-type lectin domain family 12, member A
• Calculated Molecular Weight: 31 kDa
• GenBank Accession Number: BC126291
• Gene Symbol: CLEC12A
• Gene ID (NCBI): 160364
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5QGZ9
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).