CLEC2/CLEC1B Polyclonal antibody

• 

  WB analysis of human peripheral blood platelets using 12814-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 12814-1-AP (CLEC2/CLEC1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CLEC2/CLEC1B Polyclonal Antibody for WB, ELISA

Cat No. 12814-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

1810061I13Rik, C type lectin like receptor 2, CLEC 2, CLEC1B, CLEC2, CLEC2B, PRO1384, QDED721

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Tested Applications

• Positive WB detected in: human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12814-1-AP targets CLEC2/CLEC1B in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3512

  Product name: Recombinant human CLEC1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-196 aa of BC029554

  Sequence: MQDEDGYITLNIKTRKPALVSAVMQRNYLQDENENRTGTLQQLAKRFCQYVVKQSELKGTFKGHKCSPCDTNWRYYGDSCYGFFRHNLTWEESKQYCTDMNATLLKIDNRNIVEYIKARTHLIRWVGLSRQKSNEVWKWEDGSVISENMFEFLEDGKGNMNCAYFHNGKMHPTFCENKHYLMCERKAGMTKVDQLP

• Full Name: C-type lectin domain family 1, member B
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 32-38 kDa
• GenBank Accession Number: BC029554
• Gene Symbol: CLEC2
• Gene ID (NCBI): 51266
• RRID: AB_3085400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P126
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type lectin-like receptor 2 (CLEC2, also known as CLEC1B) is a type II transmembrane receptor of the C-type lectin superfamily, which are characterized by one or more C-type lectin-like domains (CTLDs) (PMID: 10671229; 34177942). CLEC2 contains a single YXXL/hemi-ITAM (immuno-receptor tyrosine-based activation motif) within its cytoplasmic domain. CLEC-2 is highly expressed on platelets and megakaryocytes and at low levels on other hematopoietic cells, including dendritic cells, neutrophils, monocytes, and macrophages (PMID: 21728173). The first identified ligand for CLEC2 was the platelet-aggregating snake venom protein rhodocytin, and podoplanin has been established as an endogenous ligand for CLEC2 (PMID: 26151067). CLEC2 is involved in thrombosis/hemostasis, tumor metastasis, and lymphangiogenesis (PMID: 20525685). CLEC2 can be glycosylated and has been detected as 32- and 40-kDa forms in platelets with varying degrees of glycosylation (PMID: 16174766; 25150298; 21781241).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		EMBO Mol Med; Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation; Authors - Wei Jiang
human	IHC	Int J Gen Med; CLEC1B is a Promising Prognostic Biomarker and Correlated with Immune Infiltration in Hepatocellular Carcinoma.; Authors - Xiaoliang Liang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Smita (Verified Customer) (07-23-2025)

Works very well for fluorescence imaging in human platelets.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 80
• Cell Tissue Type: Platelets