CLEC2D Polyclonal antibody

CLEC2D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13188-1-AP

Synonyms

CLAX, CLEC2D, Lectin like NK cell receptor, Lectin like transcript 1, LLT 1, LLT1, OCIL, Osteoclast inhibitory lectin

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Validation Data Gallery

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  WB analysis using 13188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13188-1-AP (CLEC2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13188-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13188-1-AP (CLEC2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, Jurkat cells, Saos-2 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13188-1-AP targets CLEC2D in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLEC2D fusion protein Ag3862
• Full Name: C-type lectin domain family 2, member D
• Calculated Molecular Weight: 154 aa, 18 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC019883
• Gene Symbol: CLEC2D
• Gene ID (NCBI): 29121
• RRID: AB_2877920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC2D (C-type lectin domain family 2 member D), also known as LLT1 (lectin-like transcript 1) or OCIL (Osteoclast inhibitory lectin), is a single-pass type II membrane protein expressed by hematopoietic cells and osteoblasts (PMID: 21930700; 14753741). Its expression was found to be tightly correlated with activation on T cells, NK cells, B cells, and DCs (PMID: 21930700). CLEC2D is a ligand for the human NKR-P1A (CD161) receptor and their interaction differentially regulates NK- and T-cell functions (PMID: 16339513, 16339512). It inhibits the formation and function of osteoclasts and modulates the release of interferon-gamma by human NK cells (PMID:14753741; 20415786).