CLEC5A Polyclonal antibody

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• CLEC5A Polyclonal antibody (18248-1-AP)

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  WB analysis using 18248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18248-1-AP (CLEC5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CLEC5A Polyclonal Antibody for WB, ELISA

Cat No. 18248-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MDL-1, MDL1, MDL 1, C-type lectin superfamily member 5, C-type lectin domain family 5 member A

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Tested Applications

• Positive WB detected in: J774A.1 cells, K-562 cells, THP-1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18248-1-AP targets CLEC5A in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13025

  Product name: Recombinant human CLEC5A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 32-188 aa of BC112099

  Sequence: NKSNDGFTTTRSYGTVSQIFGSSSPSPNGFITTRSYGTVCPKDWEFYQARCFFLSTSESSWNESRDFCKGKGSTLAIVNTPEKLKFLQDITDAEKYFIGLIYHREEKRWRWINNSVFNGNVTNQNQNFNCATIGLTKTFDAASCDISYRRICEKNAK

• Full Name: C-type lectin domain family 5, member A
• Calculated Molecular Weight: 188 aa, 22 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC112099
• Gene Symbol: CLEC5A
• Gene ID (NCBI): 23601
• RRID: AB_3741950
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NY25
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type lectin domain family 5 member A (CLEC5A, also known as CLECSF5 and MDL1) is a spleen tyrosine kinase (Syk)-coupled receptor abundantly expressed by monocytes, macrophages and neutrophils (PMID: 10449773). It has a pivotal function in activating multiple aspects of innate immunity against bacterial invasion (PMID: 28824166). Both in vitro and in vivo evidence supported that CLEC5A was involved in glioblastoma pathogenesis via regulation of PI3K/Akt pathway (PMID: 30834619). It appeared as a protein band of ~36 kDa because of glycosylation, while its predicted molecular weight is 22 kDa (PMID: 25877931; 19074552).