CLEC5A Recombinant monoclonal antibody

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• CLEC5A Recombinant monoclonal antibody (86633-2-RR)

• 

  WB analysis using 86633-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86633-2-RR (CLEC5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86633-2-RR

  U-87 MG cells lysate was subjected to SDS PAGE followed by western blot with 86633-2-RR (CLEC5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86633-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86633-2-RR against Human CLEC5A were performed. The affinity constant is 1.59 nM.

CLEC5A Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86633-2-RR

Clone No.251734A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CLECSF5, C-type lectin domain family 5 member A, C-type lectin superfamily member 5, MDL 1, MDL1

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Tested Applications

• Positive WB detected in: U-251 cells, U-87 MG cells, THP-1 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86633-2-RR targets CLEC5A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  DNA

• Full Name: C-type lectin domain family 5, member A
• Calculated Molecular Weight: 188 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC112099
• Gene Symbol: CLEC5A
• Gene ID (NCBI): 23601
• RRID: AB_3724001
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NY25
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-type lectin domain family 5 member A (CLEC5A, also known as CLECSF5 and MDL1) is a spleen tyrosine kinase (Syk)-coupled receptor abundantly expressed by monocytes, macrophages and neutrophils (PMID: 10449773). It has a pivotal function in activating multiple aspects of innate immunity against bacterial invasion (PMID: 28824166). Both in vitro and in vivo evidence supported that CLEC5A was involved in glioblastoma pathogenesis via regulation of PI3K/Akt pathway (PMID: 30834619).