CLIP-115/CLIP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CLIP-115/CLIP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25088-1-AP

Synonyms

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

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Validation Data Gallery

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  WB analysis of A549 using 25088-1-AP

  WB result of CLIP-115/CLIP2 antibody (25088-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

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  WB analysis using 25088-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25088-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CYLN2 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25088-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25088-1-AP (CLIP-115/CYLN2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25088-1-AP

  IP result of anti-CLIP-115/CLIP2 (IP:25088-1-AP, 4ug; Detection:25088-1-AP 1:15000) with mouse brain tissue lysate 1080 ug.

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  IHC staining of mouse brain using 25088-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25088-1-AP (CLIP-115/CLIP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, A549 cells, rat brain tissue, mouse brain tissue, mouse cerebellum tissue, rat cerebellum tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

25088-1-AP targets CLIP-115/CLIP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLIP-115/CLIP2 fusion protein Ag18692
• Full Name: CAP-GLY domain containing linker protein 2
• Calculated Molecular Weight: 1011 aa, 112 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC150332
• Gene Symbol: CLIP2
• Gene ID (NCBI): 7461
• RRID: AB_2879890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.

Publications

Species	Application	Title
mouse	IF	Curr Biol; Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons; Authors - Sara C Sousa