CLK1 Polyclonal antibody

CLK1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20439-1-AP

Synonyms

CDC like kinase 1, CLK, CLK/STY, CLK1, STY

---

Validation Data Gallery

• 

  WB analysis using 20439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20439-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 20439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20439-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20439-1-AP (CLK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 20439-1-AP

  IP result of anti-CLK1 (IP:20439-1-AP, 4ug; Detection:20439-1-AP 1:300) with COLO 320 cells lysate 2000ug.

---

Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, HepG2 cells
• Positive IP detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20439-1-AP targets CLK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLK1 fusion protein Ag14214
• Full Name: CDC-like kinase 1
• Calculated Molecular Weight: 484 aa, 57 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC031549
• Gene Symbol: CLK1
• Gene ID (NCBI): 1195
• RRID: AB_2878686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.