CLK2 Polyclonal antibody

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  WB analysis of A549 using 11092-1-AP

  WB result of CLK2 antibody (11092-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLK2 transfected A549 cells.

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  WB analysis using 11092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11092-1-AP (CLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

CLK2 Polyclonal Antibody for WB, ELISA

Cat No. 11092-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

CDC like kinase 2, CDC-like kinase 2, EC:2.7.12.1

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Tested Applications

• Positive WB detected in: A549 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

11092-1-AP targets CLK2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1549

  Product name: Recombinant human CLK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 200-499 aa of BC014067

  Sequence: YKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLTLGEALQHPFFARLRAEPPNKLWDSSRDISR

• Full Name: CDC-like kinase 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC014067
• Gene Symbol: CLK2
• Gene ID (NCBI): 1196
• RRID: AB_2082254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49760
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma; Authors - Yinghao Wang
human	WB	Genes Cancer; PMID: 26124922; Authors - Yongshuai Jin
human	WB	Antiviral Res; Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3; Authors - Tan Ming ; KD Validated
mouse,human	WB	Bioorg Chem; A novel selenium nanocomposite modified by AANL inhibits tumor growth by upregulating CLK2 in lung cancer; Authors - Yueyang Zhang
mouse	WB,IF	Cell Signal; CLK2-SOX3 combination promotes choroidal neovascularization by SGLT1 inducing endothelial cell metabolic reprogramming; Authors - Rong Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Excellent antibody for WB: clear signal and low background noise

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat

CLK2 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:11092-1-AP)