CLN5 Recombinant antibody

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  WB analysis using 85378-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85378-1-RR (CLN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 85378-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLN5 antibody (85378-1-RR, Clone: 242812H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CLN5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85378-1-RR

Clone No.242812H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Ceroid lipofuscinosis neuronal protein 5, BMPS, BMP synthase CLN5, Bis(monoacylglycero)phosphate synthase CLN5, secreted form, Bis(monoacylglycero)phosphate synthase CLN5

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Tested Applications

• Positive WB detected in: LNCaP cells, HepG2 cells, A549 cells, HEK-293T cells, NIH/3T3 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85378-1-RR targets CLN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLN5 fusion protein Ag18175
• Full Name: ceroid-lipofuscinosis, neuronal 5
• Calculated Molecular Weight: 407 aa, 46 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC153154
• Gene Symbol: CLN5
• Gene ID (NCBI): 1203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75503
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CLN5 (ceroid lipofuscinosis neuronal protein 5) protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. CLN5 plays a role in maintaining an acidic environment in the lysosomes, a critical feature for a functional lysosome (PMID: 38236309). Moreover, CLN5-deficient neuronal progenitor cells showed reduced thioesterase activity, confirming the live cell function of CLN5 in setting S-depalmitoylation levels (PMID: 35427157).