CLOCK Recombinant antibody, PBS Only

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  WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82829-1-RR (same clone as 82829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82829-1-RR (same clone as 82829-1-PBS)

  WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells. This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82829-1-RR (same clone as 82829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82829-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82829-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82829-1-RR against Human CLOCK were performed. The affinity constant is below 1 pM.

CLOCK {ptg:Brand} Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 82829-1-PBS

Clone No.1I23

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

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Product Information

82829-1-PBS targets CLOCK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLOCK fusion protein Ag30011
• Full Name: clock homolog (mouse)
• Calculated Molecular Weight: 846 aa, 95 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC041878
• Gene Symbol: CLOCK
• Gene ID (NCBI): 9575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15516
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).