CLOCK Recombinant antibody

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  WB analysis of HeLa using 82829-1-RR

  WB result of CLOCK antibody (82829-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLOCK transfected HeLa cells.

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  WB analysis using 82829-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82829-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82829-1-RR (CLOCK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 82829-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 82829-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82829-1-RR (CLOCK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82829-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82829-1-RR against Human CLOCK were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

CLOCK Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 82829-1-RR

Clone No.1I23

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1I23, bHLHe8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, EC:2.3.1.48

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, HEK-293 cells, A431 cells, PC-3 cells, NIH/3T3 cells, rat testis tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82829-1-RR targets CLOCK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLOCK fusion protein Ag30011
• Full Name: clock homolog (mouse)
• Calculated Molecular Weight: 846 aa, 95 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC041878
• Gene Symbol: CLOCK
• Gene ID (NCBI): 9575
• RRID: AB_3086549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15516
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).