Tested Applications
| Positive WB detected in | mouse testis tissue, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14746-1-AP targets CLP1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6479 Product name: Recombinant human CLP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 123-425 aa of BC000446 Sequence: TDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK Predict reactive species |
| Full Name | CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 41 kDa, 48 kDa |
| GenBank Accession Number | BC000446 |
| Gene Symbol | CLP1 |
| Gene ID (NCBI) | 10978 |
| RRID | AB_2878076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92989 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLP1, also named as HEAB, is a 425 amino acid protein, which belongs to the Clp1 family. CLP1 as a polynucleotide kinase, that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. Human CLP1 is a component of the mRNA 3'end cleavage and polyadenylation machinery4,5 and studies in yeast have postulated a function for CLP1 in coupling mRNA 3' end processing with RNA Pol II transcriptional termination. CLP1 exists 2 isoforms with MV 41 kDa and 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLP1 antibody 14746-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CLP1 Polyclonal antibody (Cat# 14746-1-AP) has 2 total citations. These appear in Molecular Cell (2024) and Nucleic Acids Research (2016). The cited research spans RNA modification and neuroblastoma differentiation (m6Am/PCF11) and transcriptional regulation of RNA Polymerase II (P-TEFb-mediated promoter-proximal pausing release). Both studies utilized the antibody for Western blot analysis in human samples.
| Species | Application | Title |
|---|---|---|
Mol Cell m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation | ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The reviewer tested it at a 1:1000 dilution on HeLa total cell extracts and reported that it consistently detects the target protein. Overall, the feedback highlights reliable and reproducible WB performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (09-12-2025) | Consistently detects the target protein in WB. Tested on HeLa total cell extracts
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