CLP1 Polyclonal antibody

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  WB analysis of mouse testis using 14746-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14746-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CLP1 Polyclonal Antibody for WB, ELISA

Cat No. 14746-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CLP1, hClp1, HEAB, Polynucleotide kinase Clp1

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14746-1-AP targets CLP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6479

  Product name: Recombinant human CLP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 123-425 aa of BC000446

  Sequence: TDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK

• Full Name: CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 41 kDa, 48 kDa
• GenBank Accession Number: BC000446
• Gene Symbol: CLP1
• Gene ID (NCBI): 10978
• RRID: AB_2878076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92989
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLP1, also named as HEAB, is a 425 amino acid protein, which belongs to the Clp1 family. CLP1 as a polynucleotide kinase, that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. Human CLP1 is a component of the mRNA 3'end cleavage and polyadenylation machinery4,5 and studies in yeast have postulated a function for CLP1 in coupling mRNA 3' end processing with RNA Pol II transcriptional termination. CLP1 exists 2 isoforms with MV 41 kDa and 48 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation; Authors - Huihui An
		Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Consistently detects the target protein in WB. Tested on HeLa total cell extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

CLP1 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:14746-1-AP)