Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15743-1-AP targets CLPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8165 Product name: Recombinant human CLPB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 359-707 aa of BC006404 Sequence: AAIRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKITISKNFKENVIRPILKAHFRRDEFLGRINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLLWDREVADVLVDGYNVHYGARSIKHEVERRVVNQLAAAYEQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRLPKLRLEIIDKDSKTRRLDIRAPLHPEKVCNTI Predict reactive species |
| Full Name | ClpB caseinolytic peptidase B homolog (E. coli) |
| Calculated Molecular Weight | 707 aa, 79 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC006404 |
| Gene Symbol | CLPB |
| Gene ID (NCBI) | 81570 |
| RRID | AB_2847900 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H078 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CLPB encodes a protein of 707 aa with a predicted molecular mass of approximately 79 kDa, presenting two conserved ATP-binding domains. CLPB is a member of the AAA+ protein family. This potential internal translation initiation site suggests that a smaller form of ClpB, with a theoretical molecular mass of approximately 58 kDa, may be produced. (PMID: 25597510, PMID: 255957260)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CLPB antibody 15743-1-AP | Download protocol |
| IHC protocol for CLPB antibody 15743-1-AP | Download protocol |
| WB protocol for CLPB antibody 15743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CLPB Polyclonal antibody (Cat# 15743-1-AP) has 10 citations published in journals including Nature Metabolism, Nature Communications, Science Advances, Apoptosis, Cell Reports, International Journal of Molecular Sciences, iScience, Biochimica et Biophysica Acta, and eLife. Research fields span mitochondrial biology, protein disaggregation, oxidative phosphorylation, calcium signaling, ferroptosis, apoptosis, proteostasis, Ménière's disease, asphyxia diagnostics, and spermatogenesis.
| Species | Application | Title |
|---|---|---|
Nat Commun Dependence of mitochondrial calcium signalling and dynamics on the disaggregase, CLPB
| ||
Sci Adv Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding | ||
Apoptosis Mitochondrial CLPB is a pro-survival factor at the onset of granulocytic differentiation of mouse myeloblastic cells | ||
Cell Rep An updated inventory of genes essential for oxidative phosphorylation identifies a mitochondrial origin in familial Ménière's disease | ||
Int J Mol Sci Exploring Potential Diagnostic Biomarkers for Mechanical Asphyxia in the Heart Based on Proteomics Technology |
Reviews
What customers say
Both reviews are 5-star ratings for Western Blot applications. Users report strong signal with low nonspecific binding, and overall the antibody performs as expected. No issues or concerns were raised in either review.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ziwei (Verified Customer) (01-04-2026) | works as it should be.
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Ziwei (Verified Customer) (12-14-2025) | Strong signal with low nonspecific binding was observed.
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