|Positive WB detected in||mouse brain tissue, HEK-293 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
10852-1-AP targets CLTA in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CLTA fusion protein Ag1299|
|Full Name||clathrin, light chain (Lca)|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC019287|
|Gene ID (NCBI)||1211|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
|Product Specific Protocols|
|WB protocol for CLTA antibody 10852-1-AP||Download protocol|
|IHC protocol for CLTA antibody 10852-1-AP||Download protocol|
|IF protocol for CLTA antibody 10852-1-AP||Download protocol|
|IP protocol for CLTA antibody 10852-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Mol Med
Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells.
A novel binding protein of single-minded 2: the mitotic arrest-deficient protein MAD2B.
Reduced expression of programmed cell death 1 and programmed cell death ligand 1 in infiltrating inflammatory cells of lichen planus without administration of immune checkpoint inhibitors.
The mitotic arrest deficient protein MAD2B interacts with the clathrin light chain A during mitosis.
Int J Biochem Cell Biol
Autophagic lysosomal reformation depends on mTOR reactivation in H2O2-induced autophagy.
βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.
K (Verified Customer) (06-10-2021)
this Ab works well for western blotting in mouse, human and rat cells
Cristian (Verified Customer) (04-17-2019)
Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)