CLTA Polyclonal antibody

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  WB analysis using 10852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10852-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10852-1-AP (CLTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10852-1-AP

  IP result of anti-CLTA (IP:10852-1-AP, 4ug; Detection:10852-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10852-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10852-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10852-1-AP (CLTA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CLTA antibody (10852-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of HEK-293 using 10852-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10852-1-AP (CLTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CLTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10852-1-AP

Publications(22)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LCA, clathrin, light chain (Lca), Clathrin light chain A

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, K-562 cells, Raji cells, C2C12 cells, C6 cells, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 12 publications below
• IP: See 1 publications below

Product Information

10852-1-AP targets CLTA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLTA fusion protein Ag1299
• Full Name: clathrin, light chain (Lca)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC019287
• Gene Symbol: CLTA
• Gene ID (NCBI): 1211
• RRID: AB_2083025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09496
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Publications

Species	Application	Title
human	WB	Nat Commun; Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.; Authors - Tomasz J Nawara
human	WB	Proc Natl Acad Sci U S A; Clathrin light chains' role in selective endocytosis influences antibody isotype switching.; Authors - Shuang Wu ; KO Validated
human	WB	J Cell Biol; Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.; Authors - Gilles Moulay
		Small; Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.; Authors - Ji-Qian Zhang
rat	IF	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu
rat	IHC	J Bone Miner Res; Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis.; Authors - Lacruz Rodrigo S RS

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

K (Verified Customer) (06-10-2021)

this Ab works well for western blotting in mouse, human and rat cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1;1000

FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

CLTA Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10852-1-AP)