Validation Data Gallery
|Positive WB detected in||mouse brain tissue, HEK-293 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
10852-1-AP targets CLTA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CLTA fusion protein Ag1299|
|Full Name||clathrin, light chain (Lca)|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC019287|
|Gene ID (NCBI)||1211|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)
|Product Specific Protocols|
|WB protocol for CLTA antibody 10852-1-AP||Download protocol|
|IHC protocol for CLTA antibody 10852-1-AP||Download protocol|
|IF protocol for CLTA antibody 10852-1-AP||Download protocol|
|IP protocol for CLTA antibody 10852-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.
Proc Natl Acad Sci U S A
Clathrin light chains' role in selective endocytosis influences antibody isotype switching.
J Cell Biol
Alternative splicing of clathrin heavy chain contributes to the switch from coated pits to plaques.
Persistency of Enlarged Autolysosomes Underscores Nanoparticle-Induced Autophagy in Hepatocytes.
J Bone Miner Res
Adaptor protein complex 2-mediated, clathrin-dependent endocytosis, and related gene activities, are a prominent feature during maturation stage amelogenesis.
Aggregation Behavior of Nanoparticle-Peptide Systems Affects Autophagy
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.
K (Verified Customer) (06-10-2021)
this Ab works well for western blotting in mouse, human and rat cells
Cristian (Verified Customer) (04-17-2019)
Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)