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  • KD/KO Validated

CLTA Polyclonal antibody

CLTA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10852-1-AP

Synonyms

Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

10852-1-AP targets CLTA in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLTA fusion protein Ag1299
Full Name clathrin, light chain (Lca)
Calculated molecular weight 27 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC019287
Gene symbol CLTA
Gene ID (NCBI) 1211
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
WB protocol for CLTA antibody 10852-1-APDownload protocol
IHC protocol for CLTA antibody 10852-1-APDownload protocol
IF protocol for CLTA antibody 10852-1-APDownload protocol
IP protocol for CLTA antibody 10852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

Reactive oxygen species mediate angiotensin II-induced transcytosis of low-density lipoprotein across endothelial cells.

Authors - Fang Bian
ratIF

Neurogenetics

A novel binding protein of single-minded 2: the mitotic arrest-deficient protein MAD2B.

Authors - Xianfang Meng
humanIF

J Dermatol

Reduced expression of programmed cell death 1 and programmed cell death ligand 1 in infiltrating inflammatory cells of lichen planus without administration of immune checkpoint inhibitors.

Authors - Kazufumi Shirouchi
humanWB,IF,IP

PLoS One

The mitotic arrest deficient protein MAD2B interacts with the clathrin light chain A during mitosis.

Authors - Medendorp Klaas K
humanIF

Int J Biochem Cell Biol

Autophagic lysosomal reformation depends on mTOR reactivation in H2O2-induced autophagy.

Authors - Jiqian Zhang
human,mouseWB

Commun Biol

βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells.

Authors - Peng Shang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by CLTA Polyclonal antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NSC-34 cells
FH

K (Verified Customer) (06-10-2021)

this Ab works well for western blotting in mouse, human and rat cells

  • Applications: Western Blot,Immunohistochemistry
  • Primary Antibody Dilution: 1;1000
FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10852-1-AP (CLTA antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
CLTA Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10852-1-AP)