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CoraLite®647-conjugated CLTA Polyclonal antibody

CLTA Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®647 Fluorescent Dye

Cat no : CL647-10852

Synonyms

Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10852-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL647-10852 targets CLTA in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLTA fusion protein Ag1299
Full Name clathrin, light chain (Lca)
Calculated molecular weight 27 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC019287
Gene symbol CLTA
Gene ID (NCBI) 1211
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
IF protocol for CL647 CLTA antibody CL647-10852Download protocol
Standard Protocols
Click here to view our Standard Protocols