CLTB Monoclonal antibody

CLTB Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F9C2

Cat no : 66270-1-Ig

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB



Tested Applications

Positive WB detected inpig brain tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66270-1-Ig targets CLTB in WB, IHC, IF,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLTB fusion protein Ag24347
Full Name clathrin, light chain (Lcb)
Calculated molecular weight 23 kDa
Observed molecular weight 32-38 kDa
GenBank accession numberBC006457
Gene symbol CLTB
Gene ID (NCBI) 1212
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
WB protocol for CLTB antibody 66270-1-IgDownload protocol
IHC protocol for CLTB antibody 66270-1-IgDownload protocol
IF protocol for CLTB antibody 66270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Authors - Mengmeng Qin