UMP/CMP kinase Recombinant monoclonal antibody, PBS Only (Detector)

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• UMP/CMP kinase Recombinant monoclonal antibody, PBS Only (Detector) (86475-1-PBS)

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  WB analysis using 86475-1-RR (same clone as 86475-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86475-1-RR (CMPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02460-1

  The mean UMP/CMP kinase concentration was determined to be 383.79 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 348.74 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02460-1

  Sandwich ELISA standard curve of MP02460-1, Human UMP/CMP kinase Recombinant Matched Antibody Pair - PBS only. 86475-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1920. 86475-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  IHC staining of human lung cancer using 86475-1-RR (same clone as 86475-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 86475-1-RR (same clone as 86475-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86475-1-RR (same clone as 86475-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CMPK1 antibody (86475-1-RR, Clone: 251233D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02460-1

  The mean UMP/CMP kinase concentration was determined to be 134.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02460-1

  Cytometric bead array standard curve of MP02460-1, UMP/CMP kinase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86475-2-PBS. Detection antibody: 86475-1-PBS. Standard: Ag1920. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86475-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86475-1-RR against Human UMP/CMP kinase were performed. The affinity constant is below 1 pM.

UMP/CMP kinase Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86475-1-PBS

Clone No.251233D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

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Product Information

86475-1-PBS targets UMP/CMP kinase as part of a matched antibody pair:

MP02460-1: 86475-2-PBS capture and 86475-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1920

  Product name: Recombinant human CMPK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-228 aa of BC014961

  Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• RRID: AB_3744911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30085
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.