UMP/CMP kinase Recombinant monoclonal antibody

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• UMP/CMP kinase Recombinant monoclonal antibody (86475-1-RR)

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  WB analysis using 86475-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86475-1-RR (CMPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 86475-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 86475-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 86475-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CMPK1 antibody (86475-1-RR, Clone: 251233D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86475-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86475-1-RR against Human UMP/CMP kinase were performed. The affinity constant is below 1 pM.

UMP/CMP kinase Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86475-1-RR

Clone No.251233D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

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Tested Applications

• Positive WB detected in: Raji cells, Calu-3 cells, HL-60 cells, U-251 cells, HT-29 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86475-1-RR targets UMP/CMP kinase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1920

  Product name: Recombinant human CMPK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-228 aa of BC014961

  Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• RRID: AB_3744911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30085
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.