CMPK1 Recombinant monoclonal antibody, PBS Only (Capture)

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• CMPK1 Recombinant monoclonal antibody, PBS Only (Capture) (86475-2-PBS)

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  Sandwich ELISA sample test of MP02460-1

  The mean UMP/CMP kinase concentration was determined to be 383.79 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 348.74 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02460-1

  Sandwich ELISA standard curve of MP02460-1, Human UMP/CMP kinase Recombinant Matched Antibody Pair - PBS only. 86475-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1920. 86475-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Cytometric bead array sample test of MP02460-1

  The mean UMP/CMP kinase concentration was determined to be 134.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02460-1

  Cytometric bead array standard curve of MP02460-1, UMP/CMP kinase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86475-2-PBS. Detection antibody: 86475-1-PBS. Standard: Ag1920. Range: 0.781-100 ng/mL.

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  Affinity of 86475-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 86475-2-PBS against Human CMPK1 was performed. The affinity constant is 0.389 nM.

CMPK1 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86475-2-PBS

Clone No.251233H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

UMP/CMP kinase, CK, CMK, CMPK, Cytidine monophosphate kinase

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Product Information

86475-2-PBS targets CMPK1 as part of a matched antibody pair:

MP02460-1: 86475-2-PBS capture and 86475-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1920

  Product name: Recombinant human CMPK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-228 aa of BC014961

  Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30085
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.