CoraLite® Plus 488-conjugated CMPK1 Monoclonal antibody

CMPK1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D11G7

Cat no : CL488-67519

Synonyms

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-67519

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 CMPK1 antibody (CL488-67519, Clone: 2D11G7 ) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67519 targets CMPK1 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CMPK1 fusion protein Ag30084
• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 22-26 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• RRID: AB_2919489
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.