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CNN2 Monoclonal antibody

CNN2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F5

Cat no : 66244-1-Ig

Synonyms

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin



Tested Applications

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, pig stomach tissue, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66244-1-Ig targets CNN2 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNN2 fusion protein Ag23594
Full Name calponin 2
Calculated Molecular Weight 309 aa, 34 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC141818
Gene Symbol CNN2
Gene ID (NCBI) 1265
RRIDAB_2881633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

Protocols

Product Specific Protocols
WB protocol for CNN2 antibody 66244-1-IgDownload protocol
IHC protocol for CNN2 antibody 66244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols