CNOT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CNOT1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10A11

Cat no : 66507-1-PBS

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H

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Validation Data Gallery

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  WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

  WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 66507-1-Ig (same clone as 66507-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66507-1-Ig (same clone as 66507-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human cerebellum using 66507-1-Ig (same clone as 66507-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 66507-1-Ig (same clone as 66507-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66507-1-PBS targets CNOT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNOT1 fusion protein Ag5623
• Full Name: CCR4-NOT transcription complex, subunit 1
• Calculated Molecular Weight: 267 kDa
• Observed Molecular Weight: 240-250 kDa
• GenBank Accession Number: BC040523
• Gene Symbol: CNOT1
• Gene ID (NCBI): 23019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.