CNPY2, MSAP Monoclonal antibody

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• KD/KO Validated

CNPY2, MSAP Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8G11

Cat no : 66173-1-Ig

Synonyms

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

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Validation Data Gallery

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  WB analysis using 66173-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HepG2 using 66173-1-Ig

  WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

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  WB analysis of rat liver using 66173-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66173-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66173-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66173-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66173-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66173-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-mouse IgG(H+L).

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  FC experiment of HepG2 using 66173-1-Ig

  1X10^6 HepG2 cells were stained with 0.20ug CNPY2, MSAP antibody (66173-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, rat liver tissue, human placenta tissue, pig liver tissue, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66173-1-Ig targets CNPY2, MSAP in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CNPY2, MSAP fusion protein Ag6363
• Full Name: canopy 2 homolog (zebrafish)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-21 kDa
• GenBank Accession Number: BC065015
• Gene Symbol: CNPY2
• Gene ID (NCBI): 10330
• RRID: AB_2881568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2