CNTROB Monoclonal antibody

CNTROB Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67061-1-Ig
Clone No.3D12A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

3D12A4, Centrobin, Centrosomal BRCA2-interacting protein, LIP8, LYST interacting protein 8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:175-1:700
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67061-1-Ig targets CNTROB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag25447

Product name: Recombinant human CNTROB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC021134

Sequence: MATSADSPSSPLGAEDLLSDSSEPPGLNQVSSEVTSQLYASLRLSRQAEATARAQLYLPSTSPPHEGLDGFAQELSRSLSVGLEKNLKKKDGSKHIFEMESVRGQLQTMLQTSRDTAYRDPLIPGAGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPLNPPDFQGLRDALDSEHTRRKHCERHIQSLQTRVLELQQQLAVAVAADRKKDTMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEEHQAAELTRSKQQETVTRLEQSLSEAMEALNREQESARLQQRERETLEEERQALTLRLEAEQQRCCVLQEERDAARAGQLSEHRELET

Predict reactive species
Full Name centrobin, centrosomal BRCA2 interacting protein
Calculated Molecular Weight 903 aa, 101 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC021134
Gene Symbol CNTROB
Gene ID (NCBI) 116840
RRIDAB_2882371
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8N137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CNTROB antibody 67061-1-IgDownload protocol
IF protocol for CNTROB antibody 67061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Biol Cell

Binding of CEP152 to PLK4 stimulates kinase activity to promote centriole assembly

Authors - Hazal Kübra Gürkaşlar
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