COG3 Polyclonal antibody

COG3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11130-1-AP

Synonyms

COG complex subunit 3, COG3, p94, SEC34

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Validation Data Gallery

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  WB analysis using 11130-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11130-1-AP (COG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11130-1-AP

  IP result of anti-COG3 (IP:11130-1-AP, 4ug; Detection:11130-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human prostate cancer using 11130-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 11130-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 11130-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, mouse liver, rat liver
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 2 publications below

Product Information

11130-1-AP targets COG3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COG3 fusion protein Ag1610
• Full Name: component of oligomeric golgi complex 3
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC038953
• Gene Symbol: COG3
• Gene ID (NCBI): 83548
• RRID: AB_2081402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Cancer Cell; Systematic Functional Annotation of Somatic Mutations in Cancer.; Authors - Patrick Kwok-Shing Ng
human	IP	Cancer Cell; A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.; Authors - Xinxin Peng
human	WB	Cancer Cell; The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers.; Authors - Leng Han
human	WB,IP	Cancer Immunol Res; PMID: 32265228; Authors - Jiyong Liang