COG3 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• COG3 Polyclonal antibody (11130-1-AP)

COG3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11130-1-AP Copied!

Publications (9)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

COG complex subunit 3, COG3, p94, SEC34

• 

  WB analysis using 11130-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11130-1-AP (COG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 11130-1-AP

  IP result of anti-COG3 (IP:11130-1-AP, 4ug; Detection:11130-1-AP 1:500) with HeLa cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 11130-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 11130-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 11130-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for COG3 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, mouse liver, rat liver
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11130-1-AP targets COG3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1610

  Product name: Recombinant human COG3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-280 aa of BC038953

  Sequence: MAEAALLLLPEAAAERDAREKLALWDRRPDTTAPLTDRQTDSVLELKAAAENLPVPAELPIEDLCSLTSQSLPIELTSVVPESTEDILLKGFTSLGMEEERIETAQQFFSWFAKLQTQMDQDEGTKYRQMRDYLSGFQEQCDAILNDVNSALQHLESLQKQYLFVSNKTGTLHEACEQLLKEQSELVDLAENIQQKLSYFNELETINTKLNSPTLSVNSDGFIPMLAKLDDCITYISSHPNFKDYPIYLLKFKQCLSKALHLMKTYTVNTLQTLTSQLLK

• Full Name: component of oligomeric golgi complex 3
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 94 kDa
• GenBank Accession Number: BC038953
• Gene Symbol: COG3
• Gene ID (NCBI): 83548
• RRID: AB_2081402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96JB2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The COG3 Polyclonal antibody (Cat# 11130-1-AP) has 9 citations. These appear in journals including Cancer Cell, Science, Cancer Research, Cancer Immunology Research, PLoS Biology, Scientific Reports, and EMBO Journal. The associated research fields span cancer genomics and RNA editing, autophagy and neuronal resilience, PI3K resistance in head and neck cancer, PD-L1 regulation and antitumor immunity, Chlamydia parasitophorous vacuole biology, and Golgi localization with mannose 6-phosphate transport. Applications cited include Western blot and immunoprecipitation.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Cancer Cell; Systematic Functional Annotation of Somatic Mutations in Cancer.; Authors - Patrick Kwok-Shing Ng
human	WB	Cancer Cell; The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers.; Authors - Leng Han
human	IP	Cancer Cell; A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.; Authors - Xinxin Peng
human	WB	Science; Reversible suppression of autophagy in a mouse model reveals neuronal resilience; Authors - Tomoya Eguchi
		Cancer Res; Sustained Aurora Kinase B Expression Confers Resistance to PI3K Inhibition in Head and Neck Squamous Cell Carcinoma; Authors - Pooja A Shah
human	WB,IP	Cancer Immunol Res; PMID: 32265228; Authors - Jiyong Liang