Collagen Type XXV Polyclonal antibody

Collagen Type XXV Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12738-1-AP

Synonyms

AMY, CLAC, CLAC P, CLACP, COL25A1, Collagen alpha 1(XXV) chain, Collagen Type XXV, collagen, type XXV, alpha 1

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Validation Data Gallery

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  WB analysis using 12738-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12738-1-AP (Collagen Type XXV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12738-1-AP targets Collagen Type XXV in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type XXV fusion protein Ag3634
• Full Name: collagen, type XXV, alpha 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC036669
• Gene Symbol: COL25A1
• Gene ID (NCBI): 84570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen alpha-1(XXV) chain is a protein that in humans is encoded by the COL25A1 gene. COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease brains.(PMID: 11927537)