Collagen Type XXVI Polyclonal antibody

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  IHC staining of human colon using 21106-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21106-1-AP (EMID2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 21106-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using EMID2 antibody (21106-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Collagen Type XXVI Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 21106-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

COL26A1, EMID2, Collagen alpha 1(XXVI) chain, EMI domain containing 2, EMI6

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21106-1-AP targets Collagen Type XXVI in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type XXVI fusion protein Ag15382
• Full Name: EMI domain containing 2
• Calculated Molecular Weight: 441 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC110393
• Gene Symbol: EMID2
• Gene ID (NCBI): 136227
• RRID: AB_3669359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96A83
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL26A1(Collagen alpha-1(XXVI) chain), also known as EMID2. It is expected to be located in the cytoplasm and extracellular space. COL26A1 is expressed in many tissues, especially in brain tissues, including cerebral cortex, cerebellum and basal ganglia. In the single cell type, the expression of COL26A1 is mainly concentrated in excitatory and inhibitory neurons. The protein is a new member of the collagen family. Its function has not been fully demonstrated in thyroid cancer (THCA), but it is known that collagen can promote cancer progression. COL26A1 may be involved in immune-related pathways, such as CTLA4 pathway, IL-10 signaling pathway and chemokine receptor binding chemokine. The molecular weight of COL26A1 is 45 kDa. COL26A1 is significantly up-regulated in thyroid carcinoma, and its high expression is related to poor prognosis. It is also significantly related to tumor microenvironment, which may involve immune-related pathways in the process of tumor occurrence(PMID: 38197076).