Collagen Type III (N-terminal) Recombinant antibody

Collagen Type III (N-terminal) Recombinant Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2O1

Cat no : 80009-1-RR

Synonyms

COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534



Tested Applications

Positive IHC detected inhuman colon tissue, human skin cancer tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

80009-1-RR targets Collagen Type III (N-terminal) in IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type III (N-terminal) fusion protein Ag18658
Full Name collagen, type III, alpha 1
Calculated molecular weight 1466 aa, 139 kDa
Observed molecular weight 140-180 kDa
GenBank accession numberBC028178
Gene symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type III (N-terminal) antibody 80009-1-RRDownload protocol
Standard Protocols
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