CoraLite®555-conjugated Collagen Type III (N-terminal) Recombinant antibody

Collagen Type III (N-terminal) Recombinant Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2O1

Cat no : CL555-80009

Synonyms

COL3A1, Collagen alpha 1(III) chain, collagen, type III, alpha 1, EDS4A, FLJ34534

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Validation Data Gallery

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  IF Staining of human colon using CL555-80009

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®555 Collagen Type III (N-terminal) antibody (CL555-80009, Clone: 2O1 ) at dilution of 1:200.

• 

  IF Staining of human colon using CL555-80009

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®555 Collagen Type III (N-terminal) antibody (CL555-80009, Clone: 2O1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human colon tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-80009 targets Collagen Type III (N-terminal) in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Collagen Type III (N-terminal) fusion protein Ag18658
• Full Name: collagen, type III, alpha 1
• Calculated Molecular Weight: 1466 aa, 139 kDa
• Observed Molecular Weight: 140-180 kDa
• GenBank Accession Number: BC028178
• Gene Symbol: COL3A1
• Gene ID (NCBI): 1281
• RRID: AB_2919707
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody was raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen. It can't recognize mouse or rat type III collagen.