COMMD4 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84664-3-RR (same clone as 84664-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84664-3-RR (COMMD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84664-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01475-2

  Cytometric bead array standard curve of MP01475-2, COMMD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84664-3-PBS. Detection antibody: 84664-1-PBS. Standard: Ag34904. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01475-1

  Cytometric bead array standard curve of MP01475-1, COMMD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84664-3-PBS. Detection antibody: 84664-2-PBS. Standard: Ag34904. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84664-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84664-3-RR against Human COMMD4 were performed. The affinity constant is below 1 pM.

COMMD4 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 84664-3-PBS

Clone No.242175E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

242175E4, COMM domain containing 4, COMMD 4

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Product Information

84664-3-PBS targets COMMD4 as part of a matched antibody pair:

MP01475-1: 84664-3-PBS capture and 84664-2-PBS detection (validated in Cytometric bead array)

MP01475-2: 84664-3-PBS capture and 84664-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COMMD4 fusion protein Ag34904
• Full Name: COMM domain containing 4
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC068998
• Gene Symbol: COMMD4
• Gene ID (NCBI): 54939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H0A8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

COMMD4 is a protein-coding gene belonging to the COMMD family and is highly expressed in non-small cell lung cancer (NSCLC) and hepatocellular carcinoma (HCC) (PMID: 36249824). It has 3 isoforms and has been shown to control NF-κB activity and regulate the activity of cullin-RING E3 ubiquitin ligases (PMID: 32439936).