COMMD5 Monoclonal antibody

COMMD5 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1E10G6

Cat no : 67043-1-Ig

Synonyms

COMM domain containing 5, COMMD5, FLJ13008, HCARG, HT002

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Validation Data Gallery

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  WB analysis of human heart using 67043-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67043-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67043-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67043-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67043-1-Ig (COMMD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human heart tissue, rat heart tissue, MCF-7 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67043-1-Ig targets COMMD5 in WB, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: COMMD5 fusion protein Ag18464
• Full Name: COMM domain containing 5
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC003055
• Gene Symbol: COMMD5
• Gene ID (NCBI): 28991
• RRID: AB_2882357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

Publications

Species	Application	Title
human	WB	Cell; Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome; Authors - Michael D Healy
human	WB	Aging (Albany NY); TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs); Authors - Jianyun Liu