COMP Monoclonal antibody, PBS Only

COMP Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7A6

Cat no : 66793-1-PBS

Synonyms

COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5

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Validation Data Gallery

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  WB analysis of pig cartilage using 66793-1-Ig (same clone as 66793-1-PBS)

  pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66793-1-Ig (same clone as 66793-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 66793-1-Ig (same clone as 66793-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66793-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66793-1-PBS targets COMP in WB, IF, Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COMP fusion protein Ag4575
• Full Name: cartilage oligomeric matrix protein
• Calculated Molecular Weight: 757 aa, 83 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC033676
• Gene Symbol: COMP
• Gene ID (NCBI): 1311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.