COMT Polyclonal antibody

COMT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14754-1-AP

Synonyms

catechol O methyltransferase, COMT



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, human placenta, mouse liver, rat liver
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14754-1-AP targets COMT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMT fusion protein Ag6502
Full Name catechol-O-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000419
Gene symbol COMT
Gene ID (NCBI) 1312
RRIDAB_2083706
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Protocols

Product Specific Protocols
WB protocol for COMT antibody 14754-1-APDownload protocol
IHC protocol for COMT antibody 14754-1-APDownload protocol
IF protocol for COMT antibody 14754-1-APDownload protocol
IP protocol for COMT antibody 14754-1-APDownload protocol
FC protocol for COMT antibody 14754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
mouseWB

Aging (Albany NY)

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Authors - Dongdong Wang
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo
mouseWB

FASEB J

Entacapone alleviates acute kidney injury by inhibiting ferroptosis.

Authors - Jiahong Yang
humanWB,IHC

Endocrinology

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Authors - Saller S S
humanIHC

J Proteomics

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.

Authors - Prunotto Marco M