COMT Polyclonal antibody
COMT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 14754-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, K-562 cells, human placenta, mouse liver, rat liver |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse cerebellum tissue, HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
14754-1-AP targets COMT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | COMT fusion protein Ag6502 |
Full Name | catechol-O-methyltransferase |
Calculated molecular weight | 30 kDa |
Observed molecular weight | 28-30 kDa |
GenBank accession number | BC000419 |
Gene symbol | COMT |
Gene ID (NCBI) | 1312 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for COMT antibody 14754-1-AP | Download protocol |
IHC protocol for COMT antibody 14754-1-AP | Download protocol |
IF protocol for COMT antibody 14754-1-AP | Download protocol |
IP protocol for COMT antibody 14754-1-AP | Download protocol |
FC protocol for COMT antibody 14754-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Transl Psychiatry A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota. | ||
Endocrinology Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells. | ||
J Proteomics Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. | ||
Nutr Neurosci Vitamin D attenuated 6-OHDA-induced behavioural deficits, dopamine dysmetabolism, oxidative stress, and neuro-inflammation in mice. | ||
Acta Biochim Biophys Sin (Shanghai) Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene. |