COMT Polyclonal antibody

COMT Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14754-1-AP

Synonyms

catechol O methyltransferase, COMT



Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, human placenta, mouse liver, rat liver
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14754-1-AP targets COMT in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMT fusion protein Ag6502
Full Name catechol-O-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000419
Gene symbol COMT
Gene ID (NCBI) 1312
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Protocols

Product Specific Protocols
WB protocol for COMT antibody 14754-1-APDownload protocol
IHC protocol for COMT antibody 14754-1-APDownload protocol
IF protocol for COMT antibody 14754-1-APDownload protocol
IP protocol for COMT antibody 14754-1-APDownload protocol
FC protocol for COMT antibody 14754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu

Acta Biochim Biophys Sin (Shanghai)

Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene.

Authors - Liu Zhipeng Z
mouseWB

Nutr Neurosci

Vitamin D attenuated 6-OHDA-induced behavioural deficits, dopamine dysmetabolism, oxidative stress, and neuro-inflammation in mice.

Authors - Adedamola Bayo-Olugbami
humanIHC

J Proteomics

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.

Authors - Prunotto Marco M
humanWB,IHC

Endocrinology

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Authors - Saller S S
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo