COMT Polyclonal antibody

COMT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 14754-1-AP

Synonyms

catechol O methyltransferase, COMT

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Validation Data Gallery

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  WB analysis using 14754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14754-1-AP (COMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14754-1-AP

  IP Result of anti-COMT (IP:14754-1-AP, 4ug; Detection:14754-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human liver using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14754-1-AP (COMT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14754-1-AP (COMT antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14754-1-AP

  Immunofluorescent analysis of HepG2 cells, using COMT antibody 14754-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of mouse cerebellum using 14754-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (14754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse cerebellum using 14754-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (14754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 14754-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human COMT (14754-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, human placenta, mouse liver, rat liver
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse cerebellum tissue, HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

14754-1-AP targets COMT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COMT fusion protein Ag6502
• Full Name: catechol-O-methyltransferase
• Calculated molecular weight: 30 kDa
• Observed molecular weight: 28-30 kDa
• GenBank accession number: BC000419
• Gene symbol: COMT
• Gene ID (NCBI): 1312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Publications

Species	Application	Title
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	WB	FASEB J; Entacapone alleviates acute kidney injury by inhibiting ferroptosis.; Authors - Jiahong Yang
human	WB,IHC	Endocrinology; Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.; Authors - Saller S S
human	IHC	J Proteomics; Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.; Authors - Prunotto Marco M
mouse	WB	Nutr Neurosci; Vitamin D attenuated 6-OHDA-induced behavioural deficits, dopamine dysmetabolism, oxidative stress, and neuro-inflammation in mice.; Authors - Adedamola Bayo-Olugbami
		Acta Biochim Biophys Sin (Shanghai); Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene.; Authors - Liu Zhipeng Z