COMT Monoclonal antibody

COMT Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, pig, rat, mouse







Cat no : 68181-1-Ig


catechol O methyltransferase, COMT

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, pig adrenal gland tissue, human testis tissue, rat testis tissue, mouse testis tissue
Positive IF detected inmouse cerebellum tissue
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68181-1-Ig targets COMT in WB, IF, FC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

Tested Reactivity Human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COMT fusion protein Ag6502
Full Name catechol-O-methyltransferase
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC000419
Gene Symbol COMT
Gene ID (NCBI) 1312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.


Product Specific Protocols
WB protocol for COMT antibody 68181-1-IgDownload protocol
IF protocol for COMT antibody 68181-1-IgDownload protocol
FC protocol for COMT antibody 68181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols