COPE Monoclonal antibody

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  WB analysis using 66994-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66994-1-Ig (COPE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 66994-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66994-1-Ig (COPE antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

COPE Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66994-1-Ig

Clone No.2B8E8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, LNCaP cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66994-1-Ig targets COPE in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28602

  Product name: Recombinant human COPE protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-308 aa of BC017285

  Sequence: MAPPAPGPASGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVERDVFLYRAYLAQRKFGVVLDEIKPSSAPELQAVRMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGDSLECTAMTVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHLGKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLVLQYAPSA

• Full Name: coatomer protein complex, subunit epsilon
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC017285
• Gene Symbol: COPE
• Gene ID (NCBI): 11316
• RRID: AB_2882311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14579
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.