COPG Polyclonal antibody

COPG Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (3)

Cat no : 12393-1-AP

Synonyms

Coatomer subunit gamma, COPG, COPG1, Gamma coat protein, Gamma COP

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Validation Data Gallery

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  WB analysis of K-562 using 12393-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12393-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12393-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12393-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12393-1-AP

  IP Result of anti-COPG (IP:12393-1-AP, 4ug; Detection:12393-1-AP 1:2000) with K-562 cells lysate 3600ug.

• 

  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12393-1-AP (COPG antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 12393-1-AP

  Immunofluorescent analysis of HepG2 cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 12393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPG antibody (12393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 12393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPG antibody (12393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12393-1-AP

  Immunofluorescent analysis of Hela cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12393-1-AP targets COPG in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPG fusion protein Ag3070
• Full Name: coatomer protein complex, subunit gamma
• Calculated molecular weight: 874 aa, 98 kDa
• Observed molecular weight: 98 kDa
• GenBank accession number: BC020498
• Gene symbol: COPG
• Gene ID (NCBI): 22820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; Deficiency in coatomer complex I causes aberrant activation of STING signalling.; Authors - Annemarie Steiner
	IF	J Exp Med; Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation.; Authors - Masahiko Nishitani-Isa
mouse	WB	J Biol Chem; Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.; Authors - Xiaoling Li
mouse	WB	Hum Mol Genet; Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.; Authors - Desiree M Baron
human	IP,IF,WB	Sci Adv; HPV is a cargo for the COPI sorting complex during virus entry; Authors - Mara C Harwood

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RICHA (Verified Customer) (03-02-2022)

Great antibody for IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse tendon fibroblast

FH

RICHA (Verified Customer) (11-16-2020)

Excellent antibody

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

FH

RICHA (Verified Customer) (12-02-2019)

Excellent, no background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

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