Tested Applications
| Positive WB detected in | K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3070 Product name: Recombinant human COPG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-699 aa of BC020498 Sequence: RDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLERALQQYTLEPSEKPFDLKSVPLATAPMAEQRTESTPITAVKQPEKVAATRQEIFQEQLAAVPEFRGLGPLFKSSPEPVALTESETEYVIRCTKHTFTNHMVFQFDCTNTLNDQTLENVTVQMEPTEAYEVLCYVPARSLPYNQPGTCYTLVALPKEDPTAVACTFSCMMKFTVKDCDPTTGETDDEGYEDEYVLEDLEVTVADHIQKVMKLNFEAAWDEVGDEFEKEETFTLSTIKTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDILVRSRLLLLDTVTMQVTARSLEELPVDIILASVG Predict reactive species |
| Full Name | coatomer protein complex, subunit gamma |
| Calculated Molecular Weight | 874 aa, 98 kDa |
| Observed Molecular Weight | 98 kDa |
| GenBank Accession Number | BC020498 |
| Gene Symbol | COPG |
| Gene ID (NCBI) | 22820 |
| RRID | AB_2081435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y678 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COPG antibody 12393-1-AP | Download protocol |
| IHC protocol for COPG antibody 12393-1-AP | Download protocol |
| IP protocol for COPG antibody 12393-1-AP | Download protocol |
| WB protocol for COPG antibody 12393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
COPG Polyclonal antibody (Cat# 12393-1-AP) has 8 citations across journals including Nature Communications, Advanced Science, Science Advances, Journal of Experimental Medicine, EMBO Reports, Journal of Biological Chemistry, and Human Molecular Genetics. Research fields span STING/cGAS-STING signaling, HPV infection and Golgi trafficking, bladder cancer, inflammasome hyperactivation, hepatic tumorigenesis, and ALS-FUS proteomics. Applications cited include WB, IF, and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Deficiency in coatomer complex I causes aberrant activation of STING signalling. | ||
Adv Sci (Weinh) Targeting COPA to Enhance Erdafitinib Sensitivity in FGFR-Altered Bladder Cancer | ||
Sci Adv The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection.
| ||
J Exp Med Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. | ||
EMBO Rep Distinct pathogenic mutations in ARF1 allow dissection of its dual role in cGAS-STING signalling |
Reviews
What customers say
All three reviews are from the same researcher who used this antibody for Western Blot and Immunofluorescence on mouse fibroblast cells at dilutions of 1:500–1:1000. Ratings range from 4 to 5 stars. Reviewers consistently describe it as an excellent antibody, specifically praising its performance in IF with no background signal. Overall sentiment is strongly positive, indicating reliable results across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
RICHA (Verified Customer) (03-02-2022) | Great antibody for IF
|
RICHA (Verified Customer) (11-16-2020) | Excellent antibody
|
RICHA (Verified Customer) (12-02-2019) | Excellent, no background
|
