Validation Data Gallery
|Positive WB detected in||K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, NIH/3T3 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12393-1-AP targets COPG in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COPG fusion protein Ag3070|
|Full Name||coatomer protein complex, subunit gamma|
|Calculated molecular weight||874 aa, 98 kDa|
|Observed molecular weight||98 kDa|
|GenBank accession number||BC020498|
|Gene ID (NCBI)||22820|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for COPG antibody 12393-1-AP||Download protocol|
|IHC protocol for COPG antibody 12393-1-AP||Download protocol|
|IF protocol for COPG antibody 12393-1-AP||Download protocol|
|IP protocol for COPG antibody 12393-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Deficiency in coatomer complex I causes aberrant activation of STING signalling.
J Exp Med
Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation.
J Biol Chem
Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.
Hum Mol Genet
Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.
HPV is a cargo for the COPI sorting complex during virus entry
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
RICHA (Verified Customer) (03-02-2022)
Great antibody for IF
RICHA (Verified Customer) (11-16-2020)
RICHA (Verified Customer) (12-02-2019)
Excellent, no background