COPG Polyclonal antibody

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  WB analysis using 12393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12393-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 12393-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12393-1-AP (COPG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12393-1-AP

  IP result of anti-COPG (IP:12393-1-AP, 4ug; Detection:12393-1-AP 1:2000) with K-562 cells lysate 3600ug.

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  IHC staining of mouse kidney using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12393-1-AP (COPG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12393-1-AP (COPG Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 12393-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12393-1-AP (COPG antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPG antibody (12393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 12393-1-AP

  Immunofluorescent analysis of HepG2 cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of NIH/3T3 using 12393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using COPG antibody (12393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 12393-1-AP

  Immunofluorescent analysis of Hela cells, using COPG antibody 12393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

COPG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12393-1-AP

Publications(5)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Gamma-1-COP, Gamma-1-coat protein, COPG1, Coatomer subunit gamma-1

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Tested Applications

• Positive WB detected in: K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12393-1-AP targets COPG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPG fusion protein Ag3070
• Full Name: coatomer protein complex, subunit gamma
• Calculated Molecular Weight: 874 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC020498
• Gene Symbol: COPG
• Gene ID (NCBI): 22820
• RRID: AB_2081435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y678
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; Deficiency in coatomer complex I causes aberrant activation of STING signalling.; Authors - Annemarie Steiner
	IF	J Exp Med; Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation.; Authors - Masahiko Nishitani-Isa
mouse	WB	J Biol Chem; Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice.; Authors - Xiaoling Li
mouse	WB	Hum Mol Genet; Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.; Authors - Desiree M Baron
human	IP,IF,WB	Sci Adv; HPV is a cargo for the COPI sorting complex during virus entry; Authors - Mara C Harwood

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

RICHA (Verified Customer) (03-02-2022)

Great antibody for IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse tendon fibroblast

FH

RICHA (Verified Customer) (11-16-2020)

Excellent antibody

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

FH

RICHA (Verified Customer) (12-02-2019)

Excellent, no background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast