COPG Polyclonal antibody
COPG Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12393-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, A375 cells, HeLa cells, human brain tissue, human colon tissue, NIH/3T3 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, NIH/3T3 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Product Information
12393-1-AP targets COPG in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | COPG fusion protein Ag3070 |
Full Name | coatomer protein complex, subunit gamma |
Calculated molecular weight | 874 aa, 98 kDa |
Observed molecular weight | 98 kDa |
GenBank accession number | BC020498 |
Gene symbol | COPG |
Gene ID (NCBI) | 22820 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for COPG antibody 12393-1-AP | Download protocol |
IHC protocol for COPG antibody 12393-1-AP | Download protocol |
IF protocol for COPG antibody 12393-1-AP | Download protocol |
IP protocol for COPG antibody 12393-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Deficiency in coatomer complex I causes aberrant activation of STING signalling. | ||
J Exp Med Trapping of CDC42 C-terminal variants in the Golgi drives pyrin inflammasome hyperactivation. | ||
J Biol Chem Hepatic loss ofLissencephaly 1induces fatty liver and accelerates liver tumorigenesis in mice. | ||
Hum Mol Genet Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH RICHA (Verified Customer) (03-02-2022) | Great antibody for IF
|
FH RICHA (Verified Customer) (11-16-2020) | Excellent antibody
|
FH RICHA (Verified Customer) (12-02-2019) | Excellent, no background
|