COPS6 Polyclonal antibody

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  WB analysis using 10256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 10256-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 10256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of COLO 320 using 10256-1-AP

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10256-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10256-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CSN6, Signalosome subunit 6, SGN6, MOV34 34KD, JAB1-containing signalosome subunit 6

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Tested Applications

• Positive WB detected in: mouse colon tissue, 3T3-L1 cells, pig lymph node tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

• Tested Reactivity: human, mouse, rat, pig, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0331

  Product name: Recombinant human COPS6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 40-292 aa of BC002520

  Sequence: SVALHPLVILNISDHWIRMRSQEGRPVQVIGALIGKQEGRNIEVMNSFELLSHTVEEKIIIDKEYYYTKEEQFKQVFKELEFLGWYTTGGPPDPSDIHVHKQVCEIIESPLFLKLNPMTKHTDLPVSVFESVIDIINGEATMLFAELTYTLATEEAERIGVDHVARMTATGSGENSTVAEHLIAQHSAIKMLHSRVKLILEYVKASEAGEVPFNHEILREAYALCHCLPVLSTDKFKTDFYDQCNDVGLMAYL

• Full Name: COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
• Calculated Molecular Weight: 327 aa, 36 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC002520
• Gene Symbol: COPS6
• Gene ID (NCBI): 10980
• RRID: AB_2034908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L5N1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Sci; CSN6 Promotes the Migration and Invasion of Cervical Cancer Cells by Inhibiting Autophagic Degradation of Cathepsin L.; Authors - Zun Mao ; KD Validated