COPS6 Polyclonal antibody

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• KD/KO Validated

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10256-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CSN6, Signalosome subunit 6, SGN6, MOV34 34KD, JAB1-containing signalosome subunit 6

• 

  WB analysis using 10256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of 3T3-L1 using 10256-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 10256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 10256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of COLO 320 using 10256-1-AP

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10256-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse colon tissue, 3T3-L1 cells, pig lymph node tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

• Tested Reactivity: human, mouse, rat, pig, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0331

  Product name: Recombinant human COPS6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 40-292 aa of BC002520

  Sequence: SVALHPLVILNISDHWIRMRSQEGRPVQVIGALIGKQEGRNIEVMNSFELLSHTVEEKIIIDKEYYYTKEEQFKQVFKELEFLGWYTTGGPPDPSDIHVHKQVCEIIESPLFLKLNPMTKHTDLPVSVFESVIDIINGEATMLFAELTYTLATEEAERIGVDHVARMTATGSGENSTVAEHLIAQHSAIKMLHSRVKLILEYVKASEAGEVPFNHEILREAYALCHCLPVLSTDKFKTDFYDQCNDVGLMAYL

• Full Name: COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
• Calculated Molecular Weight: 327 aa, 36 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC002520
• Gene Symbol: COPS6
• Gene ID (NCBI): 10980
• RRID: AB_2034908
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L5N1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.

Publications

What published studies show

The COPS6 Polyclonal antibody (Cat# 10256-1-AP) has 1 citation. It was published in the International Journal of Biological Sciences (IF 11.7) in 2019. The cited study investigated how CSN6 promotes migration and invasion of cervical cancer cells by inhibiting autophagic degradation of Cathepsin L, placing the research in the fields of cancer biology, autophagy, and proteasome signaling.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Biol Sci; CSN6 Promotes the Migration and Invasion of Cervical Cancer Cells by Inhibiting Autophagic Degradation of Cathepsin L.; Authors - Zun Mao ; KD Validated